Tutorials on CARS/Raman microscopy
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In order to foster new cross-disciplinary collaborations in the highly active area of nonlinear optical microscopy, presentations of new scientific results will be complemented by three tutorials on CARS/Raman microscopy. The main goal of these tutorials is to provide PhD-students and Postdocs starting in the emerging field of nonlinear optical microscopy with the basic background of nonlinear optics in general and CARS/Raman microscopy in particular. These tutorials (short courses) given by experts in the respective fields will be held in the afternoon on Sunday 25 May 2008, 3 pm -6 pm.
All registered ECONOS 2008 and microCARS 2008 participants are welcome to attend the tutorials on CARS/Raman microscopy. Attendance is free and special registration is not required.
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Introduction to nonlinear optics and photonic crystal fibers (PCF's)
by Aleksei M. Zheltikov , Lomonosov Moscow State University, Moscow (Russia)
Topics included:
- basic principles of nonlinear optics (e.m. field, power series of induced polarization; polarization as source term in Maxwell's equations; phase-matching);
- the concept of the nonlinear susceptibility;
- different NLO processes (parametric/non-parametric);
- CARS signal generation: dependence on optical and molecular parameters;
- resonant and non-resonant susceptibilities;
- principles of PCF's and their use for nonlinear spectroscopy.
CARS microscopy: fundamentals and applications
by Andreas Zumbusch , Department of Physical Chemistry, University of Konstanz (Germany)
Topics included:
- basic principles of CARS under tight focusing conditions;
- the CARS microscope (instrumentation and experimental characterization);
- excitation schemes (narrow- vs. broadband pulses);
- detection schemes: forward- and epi-CARS;
- representative examples from imaging applications,
- overview of the principles of CARS microspectroscopy in the time- and frequency-domain.
Multivariate data analysis, and biological, medical and pharmaceutical applications of (linear) Raman microscopy
by Max Diem , Northeastern University, Boston (USA)
Topics included:
- principles and instrumentation for confocal Raman microscopy (CRM);
- multivariate data analysis of noisy hyperspectral data sets (hierarchical cluster analysis, principal and vortex component analysis);
- diffraction-limited Raman hyperspectral images of human cells and sub-cellular organization;
- monitoring drug uptake into cells via CRM.